An increase in enzyme or substrate concentration will - rectoria.unal.edu.co

An increase in enzyme or substrate concentration will Video

Enzyme Kinetics with Michaelis-Menten Curve - V, [s], Vmax, and Km Relationships an increase in enzyme or substrate concentration will

Factors affecting Enzyme Activity Factors affecting Enzyme Activity The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme. In nature, organisms adjust the conditions of their enzymes to produce an Optimum rate of reaction, where necessary, or they may have enzymes which are adapted to function well in extreme conditions where they live.

pH - Acidity and Basicity

Temperature Increasing temperature increases the Kinetic Energy that molecules possess. In a fluid, this means that there are more random collisions between molecules per unit time. Since enzymes catalyse reactions by randomly colliding with Substrate molecules, increasing temperature increases the rate of reaction, forming more product. However, increasing temperature also increases the Vibrational Energy that molecules have, specifically in this case enzyme molecules, which puts strain on the bonds that hold them together. As temperature increases, more bonds, especially the weaker Hydrogen and Ionic bonds, will break as a result of this strain.

an increase in enzyme or substrate concentration will

Breaking bonds within the enzyme will cause the Active Site to change shape. This change in shape means that the Active Site is less Complementary to the shape of the Substrate, so that it is less likely to catalyse the reaction. Eventually, the enzyme will become Denatured and will no longer function.

This will decrease the rate of reaction. In summary, as temperature increases, initially the rate of reaction will increase, because of increased Kinetic Energy. However, the effect of bond breaking will become greater and greater, and the rate of reaction will begin to decrease. This is different for different enzymes. Most enzymes in the human body have an Optimum Temperature of around It http://rectoria.unal.edu.co/uploads/tx_felogin/art-therapy-and-the-creative-process/annabelleigh.php from pH1 to pH Acid solutions have pH values below 7, and Basic solutions alkalis are bases have pH values above 7.

This interference causes a change in shape of the enzyme, and importantly, its Active Site.

Temperature

Different enzymes have different Optimum pH values. At the Optimum pH, the rate of reaction is at an optimum.

an increase in enzyme or substrate concentration will

Any change in pH above or below the Optimum will quickly cause a decrease in the rate of reaction, since more of the enzyme molecules will have Active Sites whose shapes are not or at least are less Complementary to the shape of their Substrate. Graph showing a typical variation of enzyme activity with acidity Small changes in pH above or below the Optimum do not cause a permanent change to the enzyme, since the bonds can be reformed.]

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