Distillation and gas chromatography - sounds temptingHow to Cite this Article Abstract Dried seeds of Monodora myristica were obtained from Oba market in Edo State, ground into fine powder and stored in an air tight plastic container at room temperature until when used. Essential oils were extracted using steam distillation and characterised using the gas chromatography-mass spectroscopy GC-MS technique. Fish samples were put in perforated polythene bags kept in plastic baskets and stored for four 4 weeks. Identification and characterisation of isolates were carried out following standard microbiological procedures. The antimicrobial activity was determined using agar well diffusion method. Data obtained were subjected to a one-way analysis of variance. The result showed that M. In view of the aforementioned, the expressed antimicrobial activity is a demonstration of the efficacy of the oil against fish spoilage organism and use in the fishery industry. Key words: Chemical compositions essential oils, antimicrobial activity, Monodora myristica, agar well diffusion method, Clarias gariepinus. distillation and gas chromatography.
In spite of its long use, there has been little prior study on the bioactivity and toxicity of the essential oils of Crassocephalum crepidioides Benth S. The antimicrobial assay was carried out using the agar diffusion method. The bacteria were three strains of Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis and Salmonella Typhi and two strains of Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa.
The main constituents of the leaves essential oil of C. The essential oils were active against all the bacterial strains but low when compared with the standard antibiotic, Gentamicin. Key words: Essential oils, toxicity, antibacterial activity, Crassocephalum crepidioides, Nigeria.]